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Growth and rVSV‐NDV production of CCX.E10 cells in shake flasks in semi‐perfusion mode, using DYN AGT (■/□), CSPR of 90 pL/(cell × day), or SCGM (▲/Δ) 73 pL/(cell × day). Infections were performed in duplicates at 1.5 × 10 7 cells/mL and 2.0 × 10 7 cells/mL at an MOI of 10 −4 . (a) Viable cell concentration (VCC) with full lines and viability in dashed lines (b) infectious virus titers determined with TCID 50 assay.

Journal: Engineering in Life Sciences

Article Title: High Cell Density Perfusion Process of Quail Cells Producing Oncolytic rVSV‐NDV

doi: 10.1002/elsc.70035

Figure Lengend Snippet: Growth and rVSV‐NDV production of CCX.E10 cells in shake flasks in semi‐perfusion mode, using DYN AGT (■/□), CSPR of 90 pL/(cell × day), or SCGM (▲/Δ) 73 pL/(cell × day). Infections were performed in duplicates at 1.5 × 10 7 cells/mL and 2.0 × 10 7 cells/mL at an MOI of 10 −4 . (a) Viable cell concentration (VCC) with full lines and viability in dashed lines (b) infectious virus titers determined with TCID 50 assay.

Article Snippet: CCX.E10 cells were cultivated in either Dynamis AGT medium (DYN) (Thermo Fisher Scientific) or suspension culture growth medium (SCGM) at 37°C, 5% CO 2 , and 185 rpm (rounds per minute) in 125 mL baffled shake flasks (Corning).

Techniques: Concentration Assay, Virus